ࡱ> XZW R2bjbj6H %EE8D%dU'"}}}&&&&&&&)+&9["}&EE' bE8& & :`%,}%02/]G% &%'0U'%R1,e3%1,3$%}" }}}&& }}}U'3}}}}}}}}} : u}Tyf[f[b,gyuRcؚyvyx~ WёP[i3u{|+R cWSyv% _>eyv% Ty AbChSOBm]~ހRyN~S Ye^YT Ng\q xvzeT Rir~ހ] z T\Oc[Ye^ Ngvt bc6e,gyut^~TNpe 07b08~uirb/gNN4N]S xvzyvN~̀ofwƋ{Nb~Q[ 1uNUSKQbSO(monoclonal antibody )wQ g~^ؚ0HeNؚ0yr_'`:_ \be@nNSS^I{yrp ]^l^(uN;Sf[uirf[TW0@wRP[uirf[b/gvSU\,$\vQ/fbSO^b/g0 bSONnS9e b/gNSяt^)R(ulWVb/gx6R[hQNnSbSOvz4x,:NUSbۏeQ4N^lucONo}Yv^(uMRof0NnSUSKQbSO nS gYOp g>fWvOp/fS'YVvMNObSOl\0RNSOQTvMQuceS^ XRoirvHeN0NLu NNnSUSKQbSOv6RY'YY(W O~USbW@x NNWVbbSOhbOpKbkeg9e bSO NQ\vQ(WNSOQv_n'`YL]TbSO CDRyibSO SDRyibSO lWV\ I{ _\ gvcNNvFm~ހT$v~ހT_USbvvՋ0vQ;NSV/fNSOyr_'`m]~ހv_ NPRir7h{US0MQuǏ zT>~ހv_S0R_'YP6R0,gyvbNNSOAbChSORy~SBm]~ހ Ǐ[b~ހT_6RYBm]~ހ8luS~ހ| N ۏNekcNnSbSOvSOYMQu_0cNnSbSOSOYMQu_vMRc/f6RYYSOYW{Qv^g ONvBm]~ހ| :NdkRy0~S0W{QBm]~ހ/f0R Nvhv͑xvzQ[0яt^egVQY gsQ NxvzbSN/f_Y FO_NS_NNN͑vxvzbg0Saito I{SsAbChSOz7hSORd/g[NTUSS^'`;pI{ glu\O(u AbChSO-N*gRSvB m]~ހ ( CD20 +) peϑ]'Y @b`SkO:N71.2 % US*N8h~ހN:N15. 5 % VdkNAbChSO_NnBm]~ހeu/fN*Nb,gNO_AbChSO\O:N!k~m]hV[ NN+T g'YϑBm]~ހ _N gTm]~ހ0Fm~ހ0 Nv~ހ0]lV~ހ0gTIl~ހI{ :N_0R~SvBm]~ހ яt^egNNc"}NNNSS gbHevel Y)R(uEsptS^StT(u[^h^y_lSdTm]~ހ (uEBvlSBm]~ހ8luSI{0~ N@b NSOAbChSOBm]~ހRySSOYW{Qb/g (WNnSbSO6RYTMQuf[W@xxvz-NwQ g͑yf[aIN /fc"}NnSUSKQbSO6RYe_vW@x]\O0Xiaocong Yu, Patricia A. McGraw, Frances S.et.al. An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies. J Immunol Methods. 2009 July 31.Martina Wiesner, Caroline Zentz, Christine Mayr,et.al. Conditional Immortalization of Human B Cells by CD40 Ligation.PLoS ONE.2008; 3(1): 1464. Ling NR. Immunoglobulin production by cultured human lymphocytes .J Immunol Methods, 2000,238, 238 (1-2) :3-15 . Tangye SG, Avery DT,Hodgkin PD. A division-linked mechanism for the rapid generateon of Ig-secreting cells from human memory B cells .J Immunol, 2003,170, 170 (1) :261-269 . Avery DT, Ellyard JI,Mackay F,et al. Increased expression of CD27 on activated human memory B cells correlates with their commitment to the plasma cell lineage .J Immunol, 2005,174, 174 (7) :4034-4042 . s gxvzW@xяt^eg,g[[_U\NÀLv~ހ0rrukUSKQbSOxvz]\O gN'Yϑ gsQm]~ހRy0~S0~ހT0T~ހSO  "*,024:>@HJXZоtbWK=-hh>*CJPJaJo(hh>*CJaJo(hhCJaJo(h5CJaJo("hh^;r5CJ$OJPJaJ$o(h~5CJ$OJPJaJ$o("hh5CJ$OJPJaJ$o(3jh>Ph>P>*CJOJQJUaJmHnHuh95CJ$OJPJaJ$o("hh95CJ$OJPJaJ$o("hh5CJ$OJPJaJ$o(h5CJ$OJPJaJ$o(h15CJ$OJPJaJ$o(2@> v  R$$ & F$1$Ifa$gd>P$IfUDdVDdWD]^`gd>P $$Ifa$$a$Zjp|~     * , 8 Ķ~seseZesh1->*CJaJo(hh^;r>*CJaJo(h>P>*CJaJo(h>PhOG>*CJaJo(%h>Ph>P5>*CJOJPJQJo(hOG>*CJaJo(hh^;rCJaJo(hh^;r5CJaJo(hh5CJaJo( h^;ro(hh>*CJPJaJo(h>*CJaJo(hh>*CJaJo(8 : < > B F L P T t v θzodVD"hUh5CJOJPJaJo(hOGh>*CJaJo(hk+>*CJaJo(h8>*CJaJo(hh8CJaJo(h85CJaJo(hh85CJaJo(hOGhOG>*CJaJo(h>P>*CJaJo(hOG>*CJaJo(h5CJaJo(hh5CJaJo(hh^;r>*CJaJo(h1->*CJaJo(hI >*CJaJo( * . @ B Z \ |   D H $\^hprz ƹƹƪƪƪƪƪƪƪƪƪƪƪƪƪƙ#h[Gh>PCJKHOJQJ^Jo( h[Gh>PCJKHOJQJ^Jh[Gh>PB*aJo(phh[Gh>PB*aJphh>PB*aJo(ph h^;ro( hUo(h5CJo(h^;r5CJo("hUh^;r5CJOJPJaJo(2=C(*.>@**+++"+.+>+N+Z+f+++,,2,D,^,p,,,,,,,--2-6-T-V-~uhj5CJo(h3h>PB*H*aJphh>Ph>PB*aJphh>Ph>PB*aJo(phUh>PB*aJo(phh^;r5CJo( h^;ro(hUh^;ro(#h[Gh>PCJKHOJQJ^Jo( h[Gh>PCJKHOJQJ^Jh>PCJKHOJQJ^Jo(.KHkdp$IfK$L$|F t634Bayt3Hkd$IfK$L$|F t634Bayt3$ & F$1$Ifa$gd>PK$$ & F$1$Ifa$gd>P "`HkdP$IfK$L$|F t634Bayt3$$1$Ifa$gd>PK$Hkd$IfK$L$|F t634Bayt3"$&(*`Gq9$IfVDWD]q^9`gdjHkd0$IfK$L$|F t634Bayt3$$1$Ifa$gd>PK$Hkd$IfK$L$|F t634Bayt3*,.0>@B*T-~~~~aRR$IfWD`gd>P$IfUDdVDdWD]^`gd>P $$Ifa$xkd$$Ifl0 T t0644 laYW{Q0bSOHeNhKmI{e.sDe0[ NT*Nb/gsvs gel ~Ǐ[E^(uTNeO9e wQYNN[[Ed\OW@x v^ShN6k'`xvzbg0 Nxvz:N,gxvz]\Ov_U\Sb NNo}YW@x0~ހ] z[[ g_U\~ހ] zvsQxvz@bvؚchYTẽ[ ,g[[] gvNhVY gẽ[ CO2W{Q{FORMA Q]\OSSW-CJ-1D y_:gLD5-2A SOƉ>f_\OLYMPUS Pnv] gIQ>f_\(NIKON OLYMPUS) >f_d\ON(NARISHIGE LEICA) 5uTN(BTX) bN(NARISHIGE LEICA ) x{N(NARISHIGE0LEICA ) _qp(NARISHIGE0LEICA ) ~4lhV(wS$NS) XlnmhVKQ-1500B ؚS_y_:gFORCE-7 m.lP05uP[)Ys^PR203 5upΘr^q{101C-3B NO)nQg Q{I{ W,gwQY_U\,gyxvzv[agN0xvzXd1. xvzvh,gyve(W[~bNAbChSOBm]~ހRy0~SSvQt[el :NNnSUSKQbSO6RYeelv^zcOW@x02. xvzQ[1 AbChSO/fSOQ[('`hV[ vQ-NvBm]~ހƖ-NR^NuS-N_ hTV g~~~T NvvS vQ gTy{|Wv~ހmBg(WNw Onc N~~yr'` ,gxvzbc"}xx0mS0RrR$cSI{elOm]~ހN~~-N8ny _0R~Sm]~ހvR6RPge02 Onc Nv~ހ0]lV~ހ0gTIl~ހ0b~~~ހI{NBm]~ހSOyN Tvyr'` ~[^h^y_vel~Sm]~ހ03 Tm]~ހTBm]~ހ'Y\veyvvc[Ye^kXQ v^Tf[ulQ^0PAGE PAGE 3"T-V-X-Z-\-^-`-b-d-f-p-r-jaaaa $$Ifa$xkd3$$Ifl0 T t0644 la$IfUDdVDdWD]^`gd>P V-\-^-`-b-f-n-p-r-x-z-|-------------......"/,/P/T/\/d/n///0:0f0h0l00000000111ݺh/}B*aJo(phh3B*aJo(phh3B*aJo(phh>Ph>PB*aJo(phh>PB*aJo(ph ho(h5CJo(h^;r5CJo( h^;ro(h>Ph>P5CJo(h/}5CJo(h>P5CJo(5r-t-v-x-z-|---..`/h00111 1$IfWD`gd>P $$Ifa$11 1"1,1012161:1<1>1H1L1P1R1V1Z1^1d1t1x111111111111111111 2$2,2.222Ƽ곪too ho(hUh9OJPJo(h9fOJPJo(h>POJPJo(h5CJo( hUo( h%7o( h>Po(hU5CJo(h95CJo(h>Ph>PCJo(h>Ph>PB*aJo(phh>PB*aJo(phh^;r5CJo( h^;ro(h>Ph>Po(h/}h>Po(* 1"1$1.10121p111~~~ooo`$IfWD`gd>P$IfWD`gd>P $$Ifa$xkd$$Ifl0 T t0644 la1111111~uuuu $IfgdOG $$Ifa$xkdY$$Ifl0 T t0644 la1111"2$2&2(2*2,2~xxooooo $Ifgd{UJ$If $$Ifa$xkd$$Ifl0 T t0644 la ,2.2p2t2v2z2|22222222yw &`#$gdPxkd$$Ifl0 T t0644 la2262^2l2n2p2r2v2x2|2~2222222222222222222222䵮Ъh3 h>Ph3h>Ph3CJOJQJaJo(hG!0JmHnHuh3 h30Jjh30JUh]jh]U h^;ro( hUo( h9o(222222222 &`#$gdP4&P 182P. A!"#$%S n$IfK$L$!vh5|#v|:V  t6,534Byt3n$IfK$L$!vh5|#v|:V  t6,534Byt3n$IfK$L$!vh5|#v|:V  t6,534Byt3n$IfK$L$!vh5|#v|:V  t6,534Byt3n$IfK$L$!vh5|#v|:V  t6,534Byt3n$IfK$L$!vh5|#v|:V  t6,534Byt3$$If!vh55T#v#vT:V l t06,55T$$If!vh55T#v#vT:V l t06,55T$$If!vh55T#v#vT:V l t06,55T$$If!vh55T#v#vT:V l t06,55T$$If!vh55T#v#vT:V l t06,55T$$If!vh55T#v#vT:V l t06,55Tyq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&|Hht lt|Hht lt|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht lt|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&|Hht ltyq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&yq~lD_&t~lȂh~yq~lD_&t~lȂh~yq~lD_&t~lȂh~yq~lD_&t~lȂh~yq~lD_&yq~lD_&t~lȂh~yq~lD_&b 2 0@P`p2( 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p8XV~PJ_HmH nHsH tHJ`J cke $1$a$ CJKH_HaJmH nHsH tH$A`$ ؞k=W[SOFi@F nfhPckee,gx6/a6 >P ckee,g Char CJKHaJPK![Content_Types].xmlj0Eжr(΢Iw},-j4 wP-t#bΙ{UTU^hd}㨫)*1P' ^W0)T9<l#$yi};~@(Hu* Dנz/0ǰ $ X3aZ,D0j~3߶b~i>3\`?/[G\!-Rk.sԻ..a濭?PK!֧6 _rels/.relsj0 }Q%v/C/}(h"O = C?hv=Ʌ%[xp{۵_Pѣ<1H0ORBdJE4b$q_6LR7`0̞O,En7Lib/SeеPK!kytheme/theme/themeManager.xml M @}w7c(EbˮCAǠҟ7՛KY,e.|,H,lxɴIsQ}#Ր ֵ+!,^$j=GW)E+& 8PK!mQtheme/theme/theme1.xmlYMoE#F{oc'vGuرhF[x=ޝzvg53NjHHzZ $ʯI)*E_N&IA!>Ǽ3xN>UWL0Č߫߿z}rx{h9T8T/χ?xY'~3S~O_|w)ӈHt=a+d NG1-RlƁ1RJwT蠯M1K"o he˓ێ½PL-|%EhY7'qP.\L=dqķ3Iofi.ñBRb-JP_pG ݢiKtdӌhFio7;7Q2ȾD>a/Q>XW ˔M_uHq)h +:Viw4rBqϫ"r!2laCbU|!8^8>ܠ,AбVt׎~ls14_=,ɬožTV G"ѦbH'.4x޵w-ϷE|F;v`b3"G 'e\fHO '$i_wp >*8i&LY%\,xҕ= r6f 3Tʅ):ªZKLs&C Mܛ0 [˫p@ע`j۽7 YHxHicT5Ar@HZAZC}i' RQ\m,zo,gQu{Y\,Nר/=L _.̇Y7C6-fSh62"5NHT[X65̫4X%Yֳ2fkhi~tCKF#b +w1m|4`!:U!p5a:~{4mmio ήc8mDJpSǹ橠V1?#Si?3E'pS2W`qB]( 08w nA[s)k8= Aa?R dڒɾcUӽ˲d)#Queb}C!&i0>4S7{z F} 4sKvUKoȳh~1jYUVH5U8Vk;֜L9ŰD ?*|FL"A3>g]dm2iVֵ褽mg ; ; H  $$$'Z8 V-1222 ""*T-r- 111,222  !#  '!!@  @ (  t  C C"`B S  ?H0(  ;  t_#`&'` < d < <*urn:schemas-microsoft-com:office:smarttagschmetcnv +011012aCFalseHasSpaceNegative NumberType SourceValueTCSCTrueUnitName       $(,:>KMRUV_~Knv %v{%?CNT^_nyz  %U]',8By",[eikdk46fjqx       $ % - 6 ? G Q W ` h r Q S k o     + -             5 6 7 < x{(-23<B$, ! $ '             5 6 < 333ss33333333333              5 6 7 <  * / A C D e              5 < 0iAJ5pNX놹0^`0o(  H\H^H`\hH) \^`\hH. \^`\hH. 4\4^4`\hH)  \ ^ `\hH. | \| ^| `\hH.  \ ^ `\hH) \^`\hH. \^`\hH. H\^H`\hH) \^`\hH. \^`\hH. 4\^4`\hH)  \^ `\hH. | \^| `\hH.  \^ `\hH) \^`\hH.0iApNֆ                  0/UI K |.UG!1`4 P6%7KE<Vb=GOGLH{UJ>P._9f^;r/}1-~8[3]3139/<.P%W7 .jU<k+*K  @4; xx$@xx*UnknownG* Times New Roman5Symbol3. * Arial;5 wiSO_GB2312;[SOSimSun;ўSOSimHeiA BCambria Math 1hI,ۆi,ۆJ,ۆ%D D !-!),.:;?]}    & 6"0000 0 000000 =@\]^([{  0 0 00000;[id 2qHX?2!xx!E:\Stone\IGEM\hT=NT\xvzReW[yvR.dot xvzReW[yvRzjUserRc~n6gnqsv{.49NUY^`n 4r   E G ` e w y }   + j m s w x z | ~ < "2BRbr0-"h2BRbrI"2BRbr  " I2 B 'R b r      k  $   :" 2 B R b r           i Z'`IZ'  Oh+'0 $0 P \ htоʵĿƻzjоʵĿƻ.dotUser4Microsoft Office Word@9+@j]@^]@]D ՜.+,0 X`lt|     !"#$&'()*+,./0123456789:;<=>?@ABCDEFHIJKLMNPQRSTUVYRoot Entry F0x4/][Data %1Table-3WordDocument6HSummaryInformation(GDocumentSummaryInformation8OCompObju  F#Microsoft Office Word 97-2003 ĵ MSWordDocWord.Document.89q